Quantification of Interbacterial Competition using Single-Cell Fluorescence Imaging

Mechanisms of Interbacterial Cooperation and Competition

Live-cell Imaging Using Fluorescence Microscopy

The ever increasing abundance of different fluorescent proteins and vital dyes that are available to researchers today make it possible to study a large variety of dynamic biological processes in live-cells [1, 2]. Many proteins of interest can be studied by expressing fluorescent fusion proteins in cells, either transiently by transfection or viral infection or permanently using transgenic ani...

Neural Imaging Using Single-Photon Avalanche Diodes

Introduction: This paper analyses the ability of single-photon avalanche diodes (SPADs) for neural imaging. The current trend in the production of SPADs moves toward the minimumdark count rate (DCR) and maximum photon detection probability (PDP). Moreover, the jitter response which is the main measurement characteristic for the timing uncertainty is progressing. Methods: The neural imaging pro...

Emerging in vivo analyses of cell function using fluorescence imaging (*).

Understanding how cells of all types sense external and internal signals and how these signals are processed to yield particular responses is a major goal of biology. Genetically encoded fluorescent proteins (FPs) and fluorescent sensors are playing an important role in achieving this comprehensive knowledge base of cell function. Providing high sensitivity and immense versatility while being m...

Quantification of integrin receptor agonism by fluorescence lifetime imaging.

Both spatiotemporal analyses of adhesion signalling and the development of pharmacological inhibitors of integrin receptors currently suffer from the lack of an assay to measure integrin-effector binding and the response of these interactions to antagonists. Indeed, anti-integrin compounds have failed in the clinic because of secondary side effects resulting from agonistic activity. Here, we ha...